Description: Physical studies of proteins, with emphasis on using single molecule methodologies and on studies of metalloproteins.
Course Website: http://www.chem.cornell.edu/pc252/chem686
Location: Baker 135
Time: MWF 12:20-01:10 PM
Topics Covered
- Physical Concepts
- Chemical structure and conformation of proteins
- Protein folding energy landscape
- Electron transfer theory
- Enzymology - Michaelis-Menten Mechanism
- Chemical Kinetics
- Experimental Methodologies
- Absorption & emission & Raman spectroscopy
- Fluorescence resonance energy transfer
- Confocal microscopy
- Total internal reflection fluorescence
- Single molecule spectroscopy
- Time correlated single photon counting
- Fluorescence correlation spectroscopy
- Atomic force microscopy
- Optical tweezers
- Magnetic Tweezers
- Protein Structure and Function
- Metalloprotein structure/function (bioinorganic chemistry)
- O2 binding and activation
- Electron transfer
- Oxygenases and oxidases
- N2O reductase
- Metallochaperones
- Metalloregulatory proteins
- GFP and variants, protein labeling
- Motor proteins: myosin, dynein, kinesin, F1-ATPase
- Protein-protein interactions
- Protein-DNA interactions